[2] State Route 341 begins at a point along US 50 in Lyon County between Mound House and Dayton, about 3.5 miles (5.6 km) to the west of the latter.

From here, SR 341 veers eastward around Silver City and Gold Hill, proceeds up the Occidental Grade and bridges over the Virginia and Truckee Railroad at the site of that line's daylighted Tunnel #5 before reconnecting with SR 342 on the south side of Virginia City.

SR 341 leaves Virginia City and travels northerly and westerly through winding sections on both sides of Geiger Summit.

The Nevada Department of Transportation refers to the entire route (except the portion between the SR 342 junctions, which is known locally as the Occidental Grade) as the Comstock Highway.

[2] SR 341 began as a toll facility constructed by Davison M. Geiger and John H. Tilton in 1862.