The western terminus of the route is at an intersection with NY 83 in Balcom Corners, a hamlet within the Chautauqua County town of Villenova.

NY 322 was assigned as part of the 1930 renumbering of state highways in New York to its current alignment.

NY 322 travels through flat, open land outside of South Dayton and through residential areas within the village.

[3] The route begins at an intersection with NY 83 and CR 87 at Balcom Corners, a hamlet within the town of Villenova.

Roughly 0.75 miles (1.21 km) east of NY 83, the route meets the west branch of Conewango Creek and turns southeastward to follow the southern bank of the waterway.

The route continues, traveling by four blocks of homes and businesses before curving eastward and entering another area dominated by open fields.