Aside from the village of Macedon at its south end and the hamlet of Ontario Center at its north terminus, NY 350 serves mostly rural areas dominated by farmland.

The conjoined routes cross over the Erie Canal and an industrial railroad spur off of the CSX Transportation-owned Rochester Subdivision before exiting the village and entering the surrounding town of Macedon.

The two routes split roughly 1.75 miles (2.82 km) north of Macedon at a junction named Cator Corners.

The route continues on, entering a slightly more forested area as it heads toward a junction with NY 286 at Atlantic Avenue.

This junction once served as Ontario Center's main business district; however, it has been replaced in purpose by the adjacent four-lane NY 104, located just 200 yards (183 m) north of Ridge Road.

NY 350 ends upon intersecting the NY 104 divided highway; however, Ontario Center Road continues north of the east–west commercial strip for 3 miles (5 km) to Lake Road near the Lake Ontario shoreline and the R. E. Ginna Nuclear Power Plant.

[12] NY 350 was extended a short distance northward in the mid-1940s to meet the new super two highway carrying US 104 between Union Hill and Sodus.