Consequently, Newnansville began to decline, and when a second railway bypassed the town in 1884, most of its residents relocated and formed the new City of Alachua.

Containing partial walls of two cemeteries, the town site was added to the National Register of Historic Places on December 4, 1974.

[citation needed] The Dell brothers (James, Simeon, and Maxey), who had earlier (during the Patriot War) visited the Alachua County area, came back to settle there sometime after 1814.

Records also show the Dell brothers staying with Edward Wanton (one of the initial founders of the Town of Micanopy) in the Fall of 1821.

[8] With the outbreak of the Second Seminole War in 1835, many residents from around the area abandoned their farms and moved to the town or nearby Fort Gilliland for refuge.

The Florida Railroad Company announced its plan to build a line from Fernandina to Cedar Key, passing several miles south of Newnansville.

The Live Oak, Tampa and Charlotte Harbor Railroad, said to be connecting Newnansville to Gainesville, bypassed the town in 1883, building its line to the south.

[8] In 1884 the town was bypassed again, when the Savannah, Florida, and Western Railroad constructed its line a mile and a half to the southwest.

This major setback, plus the lack of railway connections, led businesses and residents to move to the growing communities of Alachua and Gainesville.

[11] In 2021, the Alachua-Newnansville Community Remembrance Project identified eight victims of lynching from Newnansville: George Bibbon (1867), Cooley Johnson (1867), Willey Bradley (1868), Ceasar Sullivan (1868), Harry Hurl (1869), Joseph Hurl (1869), son of Harry Harold (1869), and William Rawls (1895).