Niccolò I Ludovisi (1610[1] – 25 December 1664), 2nd Duke of Fiano and Zagarolo, was Prince of Piombino, Marquis of Populonia, Prince of Venosa and Count of Conza, Lord di Scarlino, Populonia, Vignale, Abbadia del Fango, Suvereto, Buriano, Cerboli e Palmaiolan, and Lord prince of the Tuscan Archipelago including the islands of Elba, Montecristo, Pianosa, Gorgona, Capraia, and Isola del Giglio from 1634 until his death.
He married firstly on 30 November 1622 Donna Isabella Gesualdo (Naples, June 1611 – Rome, 8 May 1629), 4th Princess of Venosa, 9th Countess of Conza,[2] only daughter and heiress of Don Emanuele Gesualdo (Naples, 1588 - Torella, 20 August 1613), 8th Count of Conza, and wife as her first husband Martha Polyxèna, Countess zu Fürstenberg (Prague, 29 July 1588 - Atri, 31 May 1649), in turn only paternal granddaughter and heiress of Don Carlo Gesualdo, 3rd Prince of Venosa, and first wife as her third husband Donna Maria d'Avalos, and had: In 1632/1633 Niccolò married secondly Polissena de Mendoza-Appiani (?
- 1642), second daughter and heiress of Giorgio de Mendoza, Count of Binasco, and wife and maternal niece Isabella Appiani, Princess of Piombino.
- Rome, 1676), and had issue, and Camillo Francesco Maria Pamphili, 1st Prince of San Martino al Cimino and Valmontone, who married Niccolò Ludovisi's niece, Olimpia Aldobrandini.
: Für die Nuntien und Gesandten an den europäischen Fürstenhöfen, 1621-1623, Bibliothek des Deutsches Historisches Institut in Rom, Max Niemeyer Verlag, 1997, pp. 167–178.