Her stepfather announced that she had retired in March 2010, citing "lack of interest in tennis" as the primary reason, but she returned to the sport in September 2014.

Vaidišová debuted in 2003 by reaching three consecutive finals and winning the ITF tournament in Plzeň, Czech Republic.

[2][3] Vaidišová won her second title of the year at the Tashkent Open, defeating Virginie Razzano in the final.

She picked up her first Grand Slam singles victory in her Australian Open debut, by reaching the third round before falling to top seed Lindsay Davenport.

In May, Vaidišová reached her first Tier III final in Istanbul, losing to top seed Venus Williams in the championship match.

At the US Open, she reached the fourth round for the first time at a Grand Slam event before losing to Nadia Petrova.

Vaidišová's captured her first title of 2005 (and third of her career) in Seoul, defeating top seed Jelena Janković in the final without dropping a set during the week.

Vaidišová captured her sixth WTA Tour title at the Tier III event in Strasbourg in May 2006.

In June, she made a semifinal run at the French Open, her best Grand Slam performance to date.

In July, Vaidišová went 2–0 during the Czech Republic's 3–2 Fed Cup World Group play-offs loss to France.

Beginning 2007, Vaidišová reached the semifinals of the Sydney International, beating Ana Ivanovic for the first time before falling to Jelena Janković.

She went on to reach her second Grand Slam semifinal at the Australian Open, losing to eventual champion Serena Williams.

In her first grass-court tournament of the season at Eastbourne, Vaidišová lost in the quarterfinals to Justine Henin.

At Wimbledon, she lost to Ana Ivanovic in the quarterfinals after failing to convert three match points.

Moving into the indoor season, she played the Kremlin Cup, losing to Serena Williams in the quarterfinals.

The next week in Zürich, Vaidišová reached the semifinals, achieving a notable victory over Jelena Janković.

The week after the Australian Open, Vaidišová won both of her Fed Cup singles matches in the tie against Slovakia.

Later in March, her stepfather and former coach Ales Kodat announced her decision to retire from her professional career at the age of 20 due to a lack of interest in tennis.

[5] She won her first match in straight sets against Sesil Karatantcheva, before losing in the second round to Johanna Konta 6–1, 1–6, 4–6.

[6] At the Monterrey Open in March, she qualified for her first WTA Tour main draw since 2010 but she drew top seed and defending champion Ana Ivanovic in the first round.

She won her first-round match against Tímea Babos in straight sets, and lost in the second round to No.

Vaidišová became engaged to fellow Czech tennis player Radek Štěpánek, who is 11 years older, in late 2007.

[17] Only main-draw results in WTA Tour, Grand Slam tournaments, Fed Cup and Olympic Games are included in win–loss records.