[17][note 3] The service was scheduled to launch on 11/12 September 2015,[19][20][21] with the prospect of expansion across further lines in subsequent years.

[22][23] Members of several unions decided to take strike action in relation to the terms and conditions being offered by London Underground, largely regarding agreements specifically over the pay deal and hours worked by new Night Tube service personnel.

Members of the National Union of Rail, Maritime and Transport Workers (RMT), Transport Salaried Staffs' Association (TSSA), and Unite officially started the first 24-hour strike at 18:30 BST on 8 July 2015, and the Associated Society of Locomotive Engineers and Firemen (ASLEF) drivers starting their 24-hour action from 21:30 BST on 8 July 2015, with disruption occurring several hours either side of the start and finish times.

[22][23] On 27 August 2015, it was announced that the start date for the Night Tube had been pushed back due to ongoing talks about contract terms between trade unions and London Underground.

[26] Following agreement of new terms by TfL and the unions, Night Tube operations were confirmed to start in the second half of 2016.

[35] However, in November 2021, Night Tube services on the Central and Victoria lines reopened for the first time since the beginning of the pandemic, after an 18-month hiatus.