[3][4][5] The agency was founded in 2018 with the purpose of promoting the use of Live2D models and streaming as opposed to the 3D and clips format popularized by Kizuna AI.

The announcement stated that accepted talents, known as Virtual Livers, would be expected to stream live to various platforms using this app.

[10] On 17 July 2018, auditions were started for a Chinese branch in Shanghai and Taipei, and in cooperation with CAPSULE Inc., would recruit from each eight people for a total of sixteen Livers.

[18][19] In November and December, auditions began for Indian and Korean branches respectively (Nijisanji IN and KR), each beginning activity in January 2020.

This announcement came in the wake of Hololive VTuber Mano Aloe's retirement after only two weeks of activity due to online harassment.

[24] In October 2020, Nijisanji privated the various Among Us streams of its member after receiving a complaint by Masuoka, a programmer who made the popular Japanese translation mod of the game.

A simultaneous commentary livestream on their English channel, hosted by Nijisanji ID members Hana Macchia and Bonnivier Pranaja, was also held.

It was made up of five male Livers: Luca Kaneshiro, Shu Yamino, Ike Eveland, Mysta Rias, and Vox Akuma.

As part of the merger, the official social media accounts of the two branches would cease operation and the ongoing audition for their latest generation for both would be cancelled.

[37][38] On 13 July 2022, Anycolor announced the next wave of Virtual Talent Academy graduates to debut directly into Nijisanji.

[40] The first English-speaking, mixed-gender wave to debut, Iluna included Maria Marionette, Kyo Kaneko, Aia Amare, Aster Arcadia, Scarle Yonaguni, and Ren Zotto.

[41] This was the second English-speaking, mixed-gender wave to debut, and included Doppio Dropscythe, Meloco Kyoran, Hex Haywire, Kotoka Torahime, Ver Vermillion, and Zaion LanZa.

On 14 December 2022, Anycolor announced the immediate graduation of Nijisanji EN Liver Yugo Asuma of Noctyx.

[42] On 23 January 2023, Nijisanji announced AR Live "Colors," a concert featuring the members of the LazuLight, Obsidiya, Ethyria, Luxiem, and Noctyx waves.

Pastel Stage would feature Finana Ryugu, Pomu Rainpuff, Elira Pendora, Rosemi Lovelock, Selen Tatsuki, Petra Gurin, Enna Alouette, Nina Kosaka, Reimu Endō, and Millie Parfait, and Vivid Stage Luca Kaneshiro, Vox Akuma, Shu Yamino, Mysta Rias, Ike Eveland, Sonny Brisko, Fulgur Ovid, Alban Knox, and Uki Violeta.

[43] On 10 March 2023, Anycolor terminated Nijisanji EN Liver Zaion LanZa of XSOLEIL, citing multiple violations of her contract and rules set out by the agency, including copyright infringement and making "offensive remarks regarding discrimination and sexual assault".

On 26 June 2023, Nijisanji EN Liver Nina Kosaka of Ethyria announced her graduation, set for 8 July 2023.

[50][51] On 23 July 2023, Nijisanji EN Liver Mysta Rias of Luxiem announced his graduation, set for 27 August 2023.

Past graduations include ZEA Cornelia, Taka Radjiman, Siska Leontyne, Amicia Michella, Azura Cecillia, Nara Haramaung, Reza Avanluna, and Hyona Eliatora.

Usa-chan Club 28 January Debut Sanbaka Beni Zuwaigani MahiLulu LvEx Chichiyama SMC-gumi Blues Posanke Tulip-gumi Orihimeboshi Aka no Soshiki Night Kingdom Mananatsu Meifu

[45][79][80] Independent VTuber Dokibird, the alternate account of the creator behind Selen,[81][82] later stated that her hospitalization was due to a suicide attempt caused by poor management and a toxic environment.

[83][45] In response to the situation, several Nijisanji EN Livers, including Enna Alouette, Fulgur Ovid, Finana Ryugu, Uki Violeta, and Meloco Kyoran, announced that they would be taking a break from their social media accounts.

[45] On 7 February Japanese time, Nijisanji released an investor relations statement claiming that the decision to terminate Selen Tatsuki would have a negligible effect on its finances.

[86][45] On 12 February, Nijisanji EN Liver Elira Pendora premiered a YouTube video addressing the situation.

They reiterated the claim that Selen had posted the cover without waiting for permission from management and that it was set to be rereleased once approved, showing a screenshot of a partial Slack conversation.

Vox and Ike expressed that they felt hurt and sad by the contents of the documents made by someone they "considered a good friend".

In particular, Vox stated that the document cited a secretly recorded voice call as evidence of favoritism within the agency.

[90][91] Dokibird expanded on her response on 14 February, apologizing for her inclusion of the recording of Vox in the document and expressed her sentiment of wanting to move on and for the public to not inquire about further details.

[92] Later that day, Anycolor CEO Riku Tazumi released a statement addressing the controversy via the Nijisanji EN Official YouTube channel.

I deeply regret this outcome and will be rethinking how our communication in English is done.He also stated the importance of Tatsuki in cultivating the growth of Nijisanji.