Her debut as Cherubino in Cortona, Italy, in 1989 made Stemme decide to follow a professional singer's career; her studies at the University College of Opera in Stockholm were completed in 1994.
In addition to two minor roles at the Royal Swedish Opera in Stockholm, she also sang Rosalinde (Die Fledermaus), Mimì (La bohème), Euridice in Gluck's Orfeo ed Euridice, and Diana (La fedeltà premiata by Haydn).
[10] Her roles[11] include Rosalinde, Mimì in La bohème, Cio-Cio San in Madama Butterfly, Turandot, Tosca, Manon Lescaut, Suor Angelica, Euridice, Katerina in Lady Macbeth of Mtsensk, the Countess in Le nozze di Figaro, Marguerite, Agathe, Marie, Nyssia (König Kandaules), Jenůfa, Marschallin, Eva, Elisabeth, Elsa, Senta, Sieglinde, Elisabeth in Tannhäuser and Isolde.
[12] In 2006, Stemme sang Maria in the premiere of Sven-David Sandström's Ordet – en passion, on March 24 in Stockholm.
Stemme also sang Brünnhilde in the 2010 season opening at La Scala[16] and in San Francisco Opera's 2011 Ring cycle.
[17] In 2015 Stemme performed at GöteborgsOperan in Gothenburg, Sweden, in a new opera by composer Hans Gefors with libretto by Kerstin Perski and directed by Keith Warner.