It was formed in 1939 as part of the Air Force airways Wing, flying transport aircraft but was disbanded after a few months.

227 Squadron RAF in 1944 and disbanded again after the end of World War II.

It formed part of the Airways Wing at Swartkop Air Station flying ex-South African Airways Junkers Ju 52/3m's but was disbanded on 1 December the same year.

The squadron was deployed flying Beaufighters and was involved in operations in Italy, Greece and Yugoslavia until it was disbanded on 10 July 1945 after the end of the war.

The squadron moved to AFB Hoedspruit on 1 January 2004 where it is currently based.