Route 321 is a collector road in the Canadian province of Nova Scotia.

It is located in Cumberland County and connects Springhill at Route 2 with Port Howe at Trunk 6.

From Port Howe it travels southward following the eastern banks of River Philip to the town of Oxford.

From there it travels southward to River Philip where it turns westward and heads to Trunk 2 in Springhill and is known locally as Main Street.

Major Intersections/Junctions in order from Port Howe to Springhill.