It was released as the second official single from the band's second studio album Odd Blood under Secretly Canadian in the United States and Mute Records in the United Kingdom on March 23, 2010.

[2] It has been included in the in-game soundtracks for Grand Theft Auto V, FIFA 11, and NBA Baller Beats and was featured in 'Poisoned Pill' a season 2 episode of The Good Wife.

The official music video for the song, lasting four minutes and six seconds, was uploaded to YouTube on March 9, 2010 and was directed by Radical Friend.

The video features a shorter edit of the song, lasting three minutes and thirty seconds, compared to the album version which lasts five minutes and twenty-three seconds.

"[4] NME placed "O.N.E" at number 90 on its list of "150 Best Tracks of the Past 15 Years".