He was rector of Prague's General Hospital and director of the Czech Institute of Pathological Anatomy.
He was a pioneer in bacteriology and studied the etiology of infectious diseases and oncology.
In 1887, Hlava authored a widely cited article entitled About Dysentery.
Due to a translation error, the article was attributed to O. Uplavici in English publications until 1938.
[2] The name of author was written in small characters, and the translator mistook O ÚPLAVICI (About dysentery in Czech) for the name of the author and PŘEDBĚŽNÁ ZPRÁVA (preliminary report) for About Dysentery.