The main venue has a capacity of 800, and the adjacent O2 Academy 2 holds 250.
The original occupant was the short-lived Marquee N1, a music and dining venue backed by Dave Stewart of Eurythmics and club promoter Mark Fuller.
[1] American punks Alkaline Trio performed on the venue's opening night.
Other notable performers who have played at the O2 Academy Islington include MELYS, Iron Butterfly, Molly Hatchet, Tesla, Dan Reed Network, Night Ranger, Isolated Islands, Bladee, Blackfoot, Queensrÿche, KISS, Kamelot, KSI, Spock's Beard, Stratovarius, Terrorvision, Stryper, Winger, Paul Gilbert, Richie Kotzen, Steve Lukather, Steve Harley and Cockney Rebel The Winery Dogs, Diamond Head, Premiata Forneria Marconi, Edguy, Symphony X, Threshold, Big Country, Magnum, The Mission, Riverside, Satyricon, FM, Train, The Damned, MaNga, Melanie C, Zebrahead, Funeral for a Friend, Hugh Cornwell, Tarja Turunen, Jello Biafra, Gackt, Girugamesh, Hikaru Utada, The Jon Spencer Blues Explosion, Coheed and Cambria, VAMPS, August Burns Red, Max Milner, The Script, The Rasmus, Tyler, The Creator, Fall Out Boy, Silversun Pickups, Yeah Yeah Yeahs, Paramore, East 17, Morbid Angel, Dimmu Borgir, Sarah Harding, Tokio Hotel, R5, My Chemical Romance (who recorded the music video for their single Planetary (Go!)
there),[2] The Rutles, Stephen Dale Petit, Matt Stevens, Mick Taylor, JoJo, James Maslow, Achilles Heel, Toploader, Roger Chapman, Road Trip and Rolling Quartz.