[5] In 1955, ONE Inc. held the ONE Midwinter Institute, the first in a series of conferences to bring together experts and community members to talk about gay and lesbian topics.

In 1957, marking the first time the Supreme Court of the United States explicitly ruled on homosexuality, ONE Inc. fought to distribute its magazine by mail, and prevailed.

ONE houses over 600 archival collections of personal papers from activists, artists and ordinary citizens, as well as records from LGBTQ political, social, educational and cultural organizations.

ONE Archives' art collection include over 4,000 paintings, drawings, works on paper, photographs, and sculptural objects, the majority of which date from the 1940s to the present.

ONE Archives also collects and houses over 3,500 posters; textiles, such as T-shirts, banners and flags; and memorabilia such as buttons, matchbooks, dolls and other three-dimensional objects.

[35] The exhibition included works by Steven F. Arnold, Don Bachardy, Claire Falkenstein, Anthony Friedkin, Rudi Gernreich, Sister Corita Kent, and Kate Millett, among many other less known or anonymous artists.

The publication included contributions by Ann Cvetkovich, Vaginal Davis, Jennifer Doyle, Jack Halberstam, Catherine Lord, Richard Meyer, Ulrike Müller, and Dean Spade.

[36] In 2017, ONE Archives collaborated with the Museum of Contemporary Art, Los Angeles to co-present Axis Mundo: Queer Networks in Chicano L.A. as MOCA's Pacific Design Center location and the ONE Gallery.

Curated by C. Ondine Chavoya and David Evans Frantz, the exhibition highlighted a generation of queer and Chicano artists, including Laura Aguilar, Mundo Meza, Roberto Gil de Montes, Joey Terrill, and Gerardo Velazquez, among others.