The Ohmi Railway Yōkaichi Line (近江鉄道八日市線, Ōmi Tetsudō Yōkaichi-sen) is a regional railway line in Shiga Prefecture operated by Ohmi Railway.

The line is 9.3 km long, connecting Yōkaichi on the Main Line in Higashiōmi to Ōmi-Hachiman on the JR West Biwako Line in Omihachiman.

In addition to local shuttle trains, the Yōkaichi Line trains include Rapid Service that connects Yōkaichi and Ōmi-Hachiman nonstop, and through trains to the Main Line to Maibara or Kibukawa.

The line was electrified at 1500 VDC in 1946.

This article about a Japanese railway line–related topic is a stub.