The zoo is specialized in animals from the European Palearctic zoogeographical region, and the Czech Republic.

[2] Karel Hartl's lecture, "Results of crossing wolves with dogs", received a lot of attention at the World Dog Show held in June 1965 in Brno and in Prague at the annual meeting of the Fédération Cynologique Internationale (FCI) and the International Cynologic Congress.

Mating a wolf, Brita, with a German Shepherd, Kurt, then created the basis of a second line.

A third line was made by joining the wolf Arga with the female dog Asta from the Sbor národní bezpečnosti (SNB).

The wolf Lejdy of Ohrada Zoo gave birth to last line of the new breed, the father of the puppies being the German Shepherd Bojar von Shottenhof.