Ohrid-Prespa Transboundary Biosphere Reserve (Macedonian: Прекуграничен биосферен резерват „Охрид-Преспа“, romanized: Prekugraničen biosferen rezervat „Ohrid-Prespa“; Albanian: Oher – Prespe, Rezerve Ndërkufitare Biosfere) is a biosphere reserve encompassing the area of Lake Ohrid and Lake Prespa, on North Macedonia and Albania.

[1] The reserve was declared in June 2014 and comprises a combination of water bodies and surrounding mountain reliefs, covering an area of 446,244.52 hectares (1,722.96 sq mi).

[2] The Ohrid-Prespa area was declared a biosphere reserve on 11 June 2014 at the UNESCO international commission session held in Jönköping, Sweden.

[2] The proposal was made by the Lake Ohrid Bilateral Secretariat, together with the UNESCO national commissions of Macedonia and Albania, as well as their respective environment ministries.

The Ohrid-Prespa Transboundary Reserve includes various ecosystems, ranging from the mountainous areas around the lakes, to the temperate sub-tropical forests found at lower altitudes around the water basins.