Its southern terminus is Oinoi, where there are connections to Athens and Thessaloniki.

It is separated from the Athens-Thessaloniki line and follows a branch of approximately 22 kilometres (14 mi).

Construction of the line had been authorized in 1889 by the law AΨΜΕ / 7-4-1889 concerning the construction and operation of the Piraeus-Larissa and Border railway line, but it was started only in 1902 after the foundation of Hellenic State Railways.

The inauguration of the line took place on 6 March 1904, along with the construction of the first 121 kilometres (75 mi) of the line, when an official dinner for 400 people was given on the premises of Chalcis railway station, with the presence of three warships, one British, one French and one Greek.

Line A3 of the Athens Suburban Railway operates on the railway, between Athens and Chalcis via Oinoi, calling at all stations except Pyrgos Vasilissis and Kato Acharnes.