The Okaloosa darter (Etheostoma okaloosae) is a species of freshwater ray-finned fish, a darter from the subfamily Etheostomatinae, part of the family Percidae, which also contains the perches, ruffes and pikeperches.

It is indigenous to freshwater streams and tributary systems in Okaloosa and Walton Counties in northwest Florida.

Ninety percent of its range is within the Eglin Air Force Base.

[1] It is found in shallow clear creeks among vegetation over sandy bottoms.

Eggs are deposited on the bed of the creek and are not guarded by an adult fish as happens in some members of this family.

[6] Because of extensive habitat improvement and efforts by the Jackson Guard at Eglin Air Force Base, the United States Fish and Wildlife Service downlisted the species from endangered to threatened status in 2011.

Fish and Wildlife Service delisted the Okaloosa darter in 2023 due to its recovery.

[7] All known habitats of the fish (tributaries of Rocky, Toms, and Boggy Bayou) are presently being affected by commercial development and/or road construction.

Okaloosa darter research at Eglin Air Force Base.

A study of Etheostoma okaloosae (Fowler) and E. edwini (Hubbs and Cannon) in Northwestern Florida, 1975-78.