The Ole Miss Rebels softball team represents the University of Mississippi in college softball at the NCAA Division I level.

The Rebels are coached by Jamie Trachsel, who will be in her first year as head coach in 2021.

Defeated Tulsa, 5–1 Lost to Oklahoma, 1–9 Defeated Wichita State, 4–0 Lost to Oklahoma, 0–3 First appearance in NCAA Tournament Defeated Southern Illinois, 8–0 Defeated Arizona State, 2–0 Defeated North Carolina, 7–2 Regional Championship Defeated Long Beach State, 9–1 Lost to Arizona State, 1–7 Defeated Long Beach State, 1–0 Lost to Arizona State, 0–9 Defeated Chattanooga, 12–0 Lost to Louisiana, 0–2 Defeated Southeast Missouri State, 10–0 Defeated Louisiana, 5–1 Defeated Louisiana, 5–4 Regional Championship Defeated Villanova, 5–1 Lost to Arizona, 6–12 Defeated Villanova, 6–2 Lost to Arizona, 6–12 Lost to Loyola Marymount, 2–4 Defeated Grand Canyon, 9–5 Defeated Loyola Marymount, 4–2 Lost to UCLA, 1–9 Defeated Baylor, 3–0 Lost to Utah, 1–7 Defeated Baylor, 9–2 Lost to Utah, 1–4 Lost to UCLA, 7–8 Lost to UCLA, 0–1 Lost to Arizona, 2–5 Lost to Arizona, 1–9 †Denotes 1st Team selection # denotes interim head coach.