Ommastrephinae is a subfamily of squids under the family Ommastrephidae.

Ommastrephinae includes the largest species of squids belonging to the family Ommastrephidae, Humboldt squid (Dosidicus gigas) which can grow to 1.5 metres (4.9 ft) in mantle length (ML).

Some species of the subfamily (notably Sthenoteuthis and Ommastrephes) are known for their behavior of leaping out of the water (hence the common name 'flying squid').

[5] The name of the subfamily, like the family itself and one of its member genera, Ommastrephes, comes from Greek ὄμμα ('eye') and -strephes ('rolling').

Eight species of squids are recognized under Ommastrephinae, divided among six genera.