One Night of Love is a 1934 American Columbia Pictures romantic musical film set in the opera world, starring Grace Moore and Tullio Carminati.

The film was directed by Victor Schertzinger and adapted from the story Don't Fall in Love, by Charles Beahan and Dorothy Speare.

Opera singer Mary Barrett (Grace Moore) leaves to study music in Milan, Italy to the disappointment of her family in New York City.

Mary thinks Giulio has gone to Lally to rekindle a romance, and so visits Bill Houston (Lyle Talbot), a longtime friend who has proposed marriage.

On the night of her debut in Madame Butterfly, Mary is too nervous to go on stage until she sees Giulio in his usual place in the prompter's box.

[2] The complete proscenium and part of the wings and seating plan of the Metropolitan Opera House were duplicated for this production and occupied the whole of Columbia's largest sound stage.

[3] Moore's recording and performance of the Un bel di aria from Madame Butterfly did not go smoothly, as she had trouble hitting the high notes.