The OSL is affiliated with and governed by the Ontario Soccer Association.

It can be considered part of Canada's fifth tier in soccer, with teams eligible to compete for the Challenge Trophy.

The following teams are taking part in the 2022 season: [2] Caledon United FC Elite Cavan FC GS United Hrvat St. Catharines Olimpia Toronto Panathinaikos Vaughan Azzurri Woodbridge Strikers AC Soccer Diavoli Burloak Titans FC Caledon United FC PW Ebony Jrs Guelph Royals JJD Sports Lakeshore United Mississauga Croatia Aurora Hearts FC Ukraine United Downsview Rams Hungaria Toronto VFC Pana 2.0

Scarboro Azzurri Blues Toronto AFC Toronto Celtic Woodbridge Strikers Bradford Wolves Clarkson Comets Croatia Norval Distinctionz SC Guelph Royals Hamilton Serbians Orangeville Athletics Peniche CC Wisla United Artemisium FC GS Vets Korean Red Devils United Markham Lightning Olympiakos SC Peterborough City Queens Park Rangers Toronto Pamir Toronto Skillz FC Distinctionz SC MJ Ebony II Georgetown Mustangs SC Toronto Sporting FC Mens York Jets Atletico Sporting Toronto Aurora Lions Newmarket United

Rustic AC Scarborough Rangers SC