The Orto Botanico dell'Università di Roma "La Sapienza" (12 hectares), also known as the Orto Botanico di Roma, is a botanical garden operated by the Sapienza University of Rome and located at Largo Cristina di Svezia 24, Rome, Italy.

It is open every day including Sundays; an admission fee is charged.

It is sited on the slopes of the Janiculum overlooking the 17th-century Palazzo Corsini, which was from 1659-1689 the residence of Queen Christina of Sweden, now the headquarters of the Accademia dei Lincei.

After the Palace became property of the Italian state, they were merged with the existing Papal gardens to create the current lay-out.

[1] Today the garden contains more than 3,000 species, with a Japanese garden, bamboo groves, and a Giardino dei Semplici (over 300 species of medicinal plants).