Glen Osborne is a borough in Allegheny County, Pennsylvania, United States, along the Ohio River.

The area that now comprises the borough was first surveyed by Nathaniel Breading following the Revolutionary War.

Early settlers of the area included Henry Pratt in 1786 and James Park in 1805.

By 1851, the Pittsburgh, Fort Wayne and Chicago Railroad was built through what was then part of Pitt and Ohio townships and a station opened in the community named "Osborne" to prevent confusion from a similarly named station.

The borough is home to Mary Roberts Rinehart Nature Park, a nature park featuring trails that wind through ravine and floodplain forest and past planted pollinator gardens.

Across the Ohio River, Glen Osborne is adjacent with the borough of Coraopolis to the south and southeast, and Moon Township to the southwest.