The Church of Our Lady of Guadalupe (Spanish: Nuestra Señora de Guadalupe), is a former parish church under the authority of the Roman Catholic Archdiocese of New York, located at 229 West 14th Street, between Seventh and Eighth Avenues, in the Chelsea section of Manhattan in New York City.

With the merger in 2003 of the Parish of Our Lady of Guadalupe with the Parish of St. Bernard, farther west at 328 West 14th Street, the function was transferred to the nearby St. Bernard Church and the church was converted to other uses.

The church also included a "side chapel, tiny balcony, and clerestory."

[1][3] The "transformation which makes Guadalupe extremely rare, if not unique, in the city spanned two decades and involved several notable architects...."[1] The AIA Guide to NYC (Fifth Edition, 2010) called it "an extraordinary brownstone conversion.... Its Iberian ancestry is expressed both in the language of its services and in its Spanish Colonial facade.

"[4] The church remained popular with the various Hispanic communities of New York, serving Spaniards, Spanish-Americans, Puerto Ricans, Mexicans and other Latin Americans.