The eastern terminus of the cycleway is at Morrison Bay Park in Ryde and heads west along dedicated bike paths, quiet streets and the river foreshore to its western terminus at Parramatta Park in Parramatta.

[2][3][4] There is a short on-road section along Lancaster Avenue Melrose Park, and elevated steel mesh boardwalks through Ermington Nature Reserve.

There used to be a further short on-road section via Pike Street and South Street Rydalmere to Subiaco Creek, but this is no longer the case since the Subiaco Creek link opened in June 2017.

In September 2016, the NSW Government and Parramatta Council announced plans to complete a missing link adjacent to Subiaco Creek in Rydalmere.

[7] In the 12 months to February 2014, between 175 and 300 cyclists used the Parramatta Valley cycleway at Rydalmere on an average weekday, with a greater number on the weekend.