He has worked in movies like Dhanyee Meye, Agnishwar, Amar Prithivi, Bagh Bondi Khela.
[2] After getting a break as a child actor in the film Maa directed by Chitta Basu in 1958 he portrayed a number of memorable roles in the 1960s.
Known to carry himself well in both comedy, serious and tragic roles, Mukherjee was at one time an automatic choice as Bengal film legend Uttam Kumar's on-screen brother or son in several Bengali movies.
[6] Mukherjee got wide recognition for his role in Tapan Sinha's Bengali movie 'Atithi' adapted from Rabindranath Tagore's short story.
The TMC lead state government had conferred Partho Mukhopadhyay with a special award for his contribution to Bengali film industry.