Paul Charles Zamecnik (November 22, 1912 – October 27, 2009) was an American scientist who played a central role in the early history of molecular biology.

Up until his death in 2009 he maintained a lab at MGH where he studied the application of synthetic oligonucleotides (antisense hybrids) for chemotherapeutic treatment of drug resistant and XDR tuberculosis in his later years.

Paul's paternal grandparents Jan Nepomucký Zámečník (1842-1915) and Konstancie Hrubecká (1843-1924) were Czech immigrants from Budičovice and Skály respectively.

His planned time in Copenhagen was cut short because of World War II—the Germans occupied Denmark from April 1940.

When the foundation merged with the University of Massachusetts Medical School in 1997, Zamecnik moved his laboratory to MGH, where he continued to work until several weeks before his death.