According to a study done in January 2017, the species G. pearlensis was significantly less abundant in the Pearl River region as compared to G. oculifera and exhibited a smaller number of reproductively mature females.

They used sequence variation of the mitochondrial control region along with the ND4 gene and found out three samples of Graptemys pearlensis constituting reciprocally monophyletic sister clades.

Basking survey data suggest that fewer than 22,000 individuals existed in the wild in 2020, with low or moderate resiliency estimates in all portions of the turtle's range.

Fish and Wildlife Service (USFWS) assessed that the Pearl River map turtle is threatened by habitat loss and degradation, take for collection and climate change.

The construction of the Ross Barnett Reservoir between 1960 and 1963 rendered a portion of the Pearl River unsuitable for occupancy by the lotic species.