There are currently eight clubs fielding a league reserves and colts team.
The six founding MFL teams in 1988 were Kwinana, Manning, Midland, Mosman Park, North Fremantle and Cockburn.
These two teams were previously competing in the Murray Districts Football League (along with Harvey Town, South Mandurah and Waroona).
The MFL gained another two teams in 1990, with the admission of South Mandurah and Waroona, who were forced to join the league when the Murray Districts Football League disbanded at the end of the 1989 season.
In 1994, Midland, Mosman Park, Manning, North Fremantle, Cockburn and Belmont left the PFL to form Division 2 of the Sunday Football League (along with a new club from Kingsley).