[3] Adult individuals that are spawning experience a decrease in body size and weight which continues after oviposition.

[6] During mating, both animals engage each other with their penises, and the one that successfully penetrates the body wall of the other becomes the male.

[6] The eggs of Peltodoris lay are spiral shaped and white-yellow in color which measure around 180μm in diameter.

[6] The larvae are chemically attracted to fulvinol-like polyacetylenes produced exclusively by their food sponges Petrosia and Haliclona, which can trigger settlement.

[3] In the wild, Peltodoris exhibits an annual life-cycle, but have been observed to live up to 15 months in the laboratory.

[3] Peltodoris relies exclusively on the sponges Petrosia ficiforms and Haliclona fulva as its food source, which is detects using chemotaxis.

There are no other species know to prey on P. ficiforms and H. fulva, due to the fulvinol-like polyacetylenes produced by the sponges as a secondary metabolite, which are known to be cytotoxic.

Peltodoris has two main defense mechanisms that have been confirmed: mantle spicules and disruptive coloration.