The Pembina Valley /ˈpɛmbɪnə/ (French: Vallée-de-la-Pembina) is an informal geographic region of the Canadian province of Manitoba.

It is named for its major geographical feature, the Pembina Valley, which runs through the southwestern part of the region.

Other important towns include Altona and Carman.

The major industries of the Pembina Valley are agriculture and manufacturing.

The region is also home to Pembina Valley Provincial Park.