Penne-d'Agenais (French pronunciation: [pɛn daʒ(ə)nɛ], literally Penne of Agenais; Occitan: Pena d'AgenésLanguedocien: Pena d’Agenés) is a commune in the Lot-et-Garonne department in south-western France.

It is a member of Les Plus Beaux Villages de France (The Most Beautiful Villages of France) Association.

Penne-d'Agenais station has rail connections to Périgueux and Agen.

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