Petra Holubová (born 12 October 1968) is a Czech former professional tennis player.

Holubová, who reached a career-high ranking of 132 in the world, competed in the qualifying draw for the 1992 US Open.

[1] Her best performances on the WTA Tour were round of 16 appearances at the Belgian Open and Palermo in 1992.

Holubová is mother of the German tennis player Julia Stusek.