[5] In doubles, she played alongside fellow Czech Kateřina Vaňková but they fell at the first hurdle to Mariana Duque Mariño and Michelle Larcher de Brito.

[8] She also made her Fed Cup debut in 2007, playing alongside Barbora Strýcová in doubles, where they lost to the Spanish team of Nuria Llagostera Vives and Virginia Ruano Pascual.

Kvitová began 2008 by upsetting Anabel Medina Garrigues in her opening match in Paris for her first ever WTA main-draw victory before falling to fourth seed Elena Dementieva.

[8] Kvitová then made her Grand Slam tournament debut appearance at the French Open, and went to the last 16 where she lost to Kaia Kanepi in three sets, having defeated Akiko Morigami, Samantha Stosur and 12th seed Ágnes Szávay en route.

[11] Kvitová started 2009 impressively by reaching her first final, at the Hobart International, having defeated Sally Peers, Alona Bondarenko, Anastasia Pavlyuchenkova, Virginie Razzano en route.

[15] She then sailed to the semifinals of Wimbledon, defeating Sorana Cîrstea, Zheng Jie, Victoria Azarenka, Caroline Wozniacki, and Kaia Kanepi en route, before falling to defending and eventual champion Serena Williams in straight sets.

Then, at the Australian Open, where second-seeded Kvitová was the favourite for the title, she defeated Vera Dushevina, Carla Suárez Navarro, Maria Kirilenko and Ana Ivanovic to reach her second consecutive quarterfinal of the tournament.

[47] The following month in Stuttgart, she defeated Schiavone and Angelique Kerber to reach the semifinals, where she lost to Sharapova in straight sets, thus snapping a long run of 27 consecutive wins indoors.

In the opening round of Fed Cup, where the Czech team faced Australia, Kvitová started with a win in singles over Jarmila Gajdošová and followed it up with a three-set victory over Samantha Stosur, saving a match point in the process.

At the Qatar Open, third-seeded Kvitová cruised into the quarterfinals with two tough three-set victories over Venus Williams and Šafářová before falling to Jelena Janković in straight sets.

[87] At the Fed Cup semifinal tie against Italy, Kvitová dispatched both Camila Giorgi and Roberta Vinci in straight sets, thus sending the Czech team into their third final in the last four years.

She then recorded a straight-set victory over Sharapova, ending a five-match losing streak against her, but then lost her bid to qualify for the semifinals when she succumbed to Caroline Wozniacki in her last round-robin match.

[130] Kvitová had a slow start in 2016 due to gastrointestinal illness,[131] going 2–4 in main draw matches for the first two months, with the wins coming over Luksika Kumkhum at the Australian Open and Barbora Strýcová in Doha.

[141] Kvitová suffered a dire grass court season as she failed to produce a winning record on the surface, falling early to Jeļena Ostapenko and Johanna Konta in Birmingham and Eastbourne respectively.

[169] Kvitová kicked off the Asian swing in Wuhan where she was the defending champion and faced local player Peng Shuai at the first hurdle, but came up short in an epic three-set match that lasted three hours and 34 minutes.

[170] At the China Open, she moved past fellow Czech Kristýna Plíšková and American Varvara Lepchenko to reach the third round where saw off fifth seed Caroline Wozniacki, in straight sets.

[190] The following week in Cincinnati, her form improved, beating Serena Williams, Mladenovic and 15th seed Elise Mertens to sail into her first semifinal of the tournament since 2012, where she was stopped by Bertens once more, this time in three sets.

[201] As the eighth seed at the Australian Open, Kvitová cruised into the quarterfinals without dropping a set, recording wins over Magdaléna Rybáriková, Irina-Camelia Begu, Belinda Bencic and Amanda Anisimova.

[217] Seeded sixth at the tournament, also her seventh appearance here, the most of any player this decade, however, Kvitová suffered a winless outing for the second year in a row, with losses to Osaka, Bencic and Barty, thus crashing out in the round-robin stage.

However, she succumbed to ninth seed Aryna Sabalenka in the championship match in straight sets, making it the tenth runner-up finish of her career, and the first time she lost consecutive finals at the Premier Mandatory/5 level.

[225][226][227] However, in spite of the suspensions, Kvitová made her unofficial return to the sport in May at an all-Czech exhibition event held in Prague, where she beat the likes of Barbora Krejčíková, Siniaková and Karolína Muchová to lift the title.

[229][230] Returning to competition in August at the Cincinnati tournament, held in New York City this year due to the ongoing COVID-19 pandemic, Kvitová lost her opening match to compatriot Marie Bouzková in three sets.

[240] Then, as the defending champion in Stuttgart, she saw off the likes of top-20 oppositions in Jennifer Brady and Maria Sakkari to reach the quarterfinals where she was stopped by Svitolina despite having led by a set, once again, and having two match points this time around.

[243] At the French Open, 11th-seeded Kvitová saved a match point in the first round against Greet Minnen en route to a win from a set down but then was forced to withdraw from the tournament due to an ankle injury sustained during media duties there.

[249] In tennis competition, she saw off Italy's Jasmine Paolini in her opener before taking her earliest loss at the event, in the hands of Belgian Alison van Uytvanck in the second round, besides being inflicted her first bagel set at the Games in the process.

[252] She then reached her third quarterfinal in Cincinnati, her most of such showings at a WTA 1000 event in North America, and best result in more than three months, where she faced Kerber but was forced to retire down a set due to a stomach issue.

[11] After yet another early loss in Indian Wells in the third round to the eventual runner-up, this time in Maria Sakkari,[265] Kvitová recorded her best result in more than six months with a third quarterfinal showing in Miami, defeating Clara Burel, Lauren Davis and 21st seed Veronika Kudermetova along the way.

[11] At the French Open, 32nd seed Kvitová scored her season's maiden win on clay when she beat Hungary's Anna Bondár in the first round, also making it her 30th victory of the tournament.

She withdrew from two events Stuttgart and Rome injured, and went winless in her other two appearances on the surface, namely, Madrid and the French Open – falling to Jule Niemeier and Elisabetta Cocciaretto respectively.

[11] Having not played at all in 2024 due to pregnancy and the birth of her first child, Kvitová announced on 3 February 2025, that she intended to return to competition later that month at the ATX Open in Austin, Texas.