Pezizomycotina is the largest subdivision of Ascomycota, containing the filamentous ascomycetes and most lichenized fungi.

This subdivision includes almost all ascus fungi that have fruiting bodies visible to the naked eye, except for the genus Neolecta, which belongs to Taphrinomycotina.

The rest of the Pezizomycotina also include the previously defined hymenial groups Discomycetes (now Leotiomycetes) and Pyrenomycetes (Sordariomycetes).

Some important groups in Pezizomycotina include: Pezizomycetes (the operculate discomycetes), Leotiomycetes (the inoperculate discomycetes), Laboulbeniomycetes, Sordariomycetes, Dothideomycetes.

Paleopyrenomycites from the Early Devonian Rhynie Chert is the oldest known fossil member of Pezizomycotina, although its position within this subdivision is unclear.