In 1641, Governor Charles Huault de Montmagny took him into his service as a soldier in the garrison of Quebec City, but especially as an interpreter and agent to the Indian tribes due to his familiarity with the Huron dialect.

His L'histoire veritable et naturelle des moeurs et productions du pays de la Nouvelle-France, vulgairement dite le Canada, a description of the flora, fauna and native societies in the region (and a significant, pioneering documentation of North American natural history) was published in Paris in 1664.

He withdrew from public office to establish his Seigneurie centred on Boucherville on the south shore of the St Lawrence River near Montreal and extending out well into the Montérégie region.

Pierre Boucher later married Jeanne Crevier, who bore 15 children; Their descendants took many names and thrived in the diaspora of New France, and many lines are still in existence today.

The eldest son, Pierre Boucher inherited the title and seigneurie of Boucherville, which had excellent land in the alluvial floodplain of the St Lawrence River.