He studied at the Faculty of Science at Charles University, under the guidance of Professor Josef Velenovský.
In 1930, he joined the National Museum, eventually becoming head of the Mycological Department, and in 1960 a corresponding member of the academy.
He was the author of many popular and scholarly publications in the field of mycology and mountain flora.
He also served as the main editor of the scientific journal Czech Mycology, and described several species of fungi.
[4] In 1936, he wrote with Dr Charles Kavina - 'Atlas des champignons de l'Europe' [5] Pilát died suddenly of cardiac failure in 1974.