The Port Perry Branch is a rail line owned and operated by the Norfolk Southern Railway in the U.S. state of Pennsylvania.

The Main Line of the Pennsylvania Railroad (PRR) was originally constructed with the goal of providing a rail link from the Eastern Seaboard to the waters of the Ohio River at Pittsburgh.

[1] In 1877, the branch was complete between Brinton's Station on the Main Line and Port Perry on the north bank of the Monongahela River.

[3] The river bridge between Port Perry and the PV&C opened the following year,[4] completing the alternate route around the congestion of the Main Line and the passenger station complex in Pittsburgh.

In the 1990s, Conrail began to use the Port Perry Branch, together with the Mon Line to Pittsburgh (including the former PV&C main line, a small portion of the Panhandle Route, and the Ohio Connecting Railroad Bridge) as a double-stack high clearance route.