Pouilley-les-Vignes (French pronunciation: [pujɛ le viɲ]) is a commune in the Doubs department in the Bourgogne-Franche-Comté region in eastern France.

The commune lies 8 km (5.0 mi) northwest of Besançon.

Fortifications were built on the surrounding heights by Jean de Chalon, Count of Burgundy in the 10th century.

The only remains are the Porte d'Orange, a gate carved from the rock, and the Baraque des Enragés, a subterranean entrance to the fort.

Until 1920, wines such as Pulsard, Pinot, and Gamay were made in the commune, but very few small vineyards remain.