Pouilly-sur-Serre (French pronunciation: [puji syʁ sɛʁ], literally Pouilly on Serre) is a commune in the Aisne department, Hauts-de-France, northern France with approximately 530 inhabitants.

The river Serre forms the northern border of the commune.

Pouilly is in the middle of a huge agricultural plain, where the fields are typically used for growing vegetables and grains.

Pouilly was the location of a leprosy facility that was part of a Laon religious order.

About 75,000 tonnes of Italian, exotic, and quenelles dishes are made each year, under the trademarks "Garbit", "Petitjean" and "Panzani".