Planned by Cartoon Network and produced by TV Tokyo, Aniplex, and Toei Animation, the series is directed by Iku Ishiguro, with Yoshio Urasawa handling series scripts, Miho Shimogasa (character designer of Cutie Honey Flash and Ultra Maniac and one of the animation directors of Sailor Moon) designing the characters and Hiroshi Nakamura and Taichi Master composing the music.

As production occurred in Japan, The Powerpuff Girls original series creator Craig McCracken was not actively involved with the project.

Despite owning the IP rights to it, the anime series never aired on Cartoon Network or its sister channel Boomerang in the United States.

Numerous rays of black light engulf people, animals, and objects to transform them into evil monsters who want to take over Tokyo City, such as Mojo Jojo, Fuzzy Lumpkins, Princess Himeko, Sedusa, the Gangreen Gang and the Amoeba Boys.

The soundtrack consists of TV size versions of most of the series theme songs, the series score by composers Taichi Master and Hiroshi Nakamura presented in the form of a party mix and character songs performed by Japanese voice actresses Emiri Katō, Nami Miyahara and Machiko Kawana who voiced the Powerpuff Girls Z.

A manga adaptation illustrated by Shiho Komiyuno [ja] was published in Shueisha's Ribon magazine between July 3, 2006,[4] and June 2, 2007.