Princess Anastasia was born at the village of Martkopi in Kakheti in 1763 as the twelfth child into the family of Heraclius and Darejan.

As she did not receive a dowry from her father, at the time of the Russian annexation of Georgia in 1801, Anastasia's possessions were limited to a garden near Tiflis granted by her half-brother, the late king George XII.

[2] In 1802, she was suspected by the Russians of being involved in facilitating the communication between her mother Queen Dowager Darejan in Tiflis and her fugitive anti-Russian brothers, Iulon and Parnaoz, in Imereti.

[3] After the establishment of the Russian rule, Anastasia's husband entered the imperial civil service as a Collegiate Counsellor.

Already a widow, Anastasia moved to St. Petersburg in 1825, bringing her younger son, Giorgi, for further education in the imperial capital.