Professor Achilles Milo first appeared in Detective Comics #247 and was created by Bill Finger and Sheldon Moldoff.

Throughout his appearances, he transforms Anthony Lupus into a werewolf, takes control of Arkham Asylum, obtains the Silver Wheel of Nyorlath, contributes to the creation of Batman's Zur-En-Arrh personality, and gives the Human Flame innate pyrokinetic abilities.

[1] Professor Milo is an expert in chemistry and alchemy.

[2] Years prior, he experimented on a faulty teleportation device that seemingly killed his colleague Leo Scarlett until Batman discovered Milo's work and defeated him.

Professor Milo appears as a non-playable character in Batman: Arkham Underworld, voiced again by Armin Shimerman.