The reserve and its surroundings, including the Eastern Creek landscape, are of significant cultural worth to the Darug people, also having weighty scientific and educational values.

[3] Other plant species in the area include, Forest Red Gum (E. Tereticornis), Spotted Gum (Corymbia maculata), Thin-leaf stringybark (E. eugenioides) narrow-leaved ironbark (E. Crebra), Blackthorn (Bursaria spinosa), Pimelea spicata, Acacia pubescens, Hickory (Acacia implexa), Native Indigo (Indigofera australis) and Dodonaea viscosa.

A rapid flora survey of Prospect Reservoir (approximately 1 km North from spoil site) revealed over fifty native species.

[8] Less than 13% of CPW remains and a high proportion of this figure is heavily degraded through weed invasion, rubbish dumping, illegal vehicle use and overgrazing.

Mammals such as wombats, echidnas and eastern grey kangaroos are listed as recent sightings in the National Park species atlas.

[3] Animals species in the reserve include a few bat, bird, and amphibians species, such as, Petaurus norfolcensis, Litoria aurea, Pteropus poliocephalus, Myotis macropus, Petaurus norfolcensis, Mormopterus norfolkensis, Falsistrellus tasmaniensis, Scoteanax rueppellii, Meridolum corneovirens, Lathamus discolor, Hieraaetus morphnoides, Tyto novaehollandiae and Daphoenositta chrysoptera.

[11] On the western slopes of George Maunder Lookout lies the Prospect dolerite intrusion, which is Sydney's largest body of igneous rock, that formed in the Early Jurassic after a volcanic activity where hot magmatic fluids moved through developing pegmatite and depositing prehnite, calcite and other secondary minerals found in the upper part of the intrusion.

[17] There is also a cycleway leading to the reserve that is accessed from Prospect Highway, at the park's southern vicinity, which trails just south of the reservoir to Westlink M7.

[19] Prospect Reservoir and its nature reserve was listed on the New South Wales State Heritage Register on 18 November 1999 having satisfied the following criteria.