The Protestant Church in Hesse and Nassau (German: Evangelische Kirche in Hessen und Nassau, EKHN) is a United Protestant church body in the German federal states of Hesse and Rhineland-Palatinate.

The EKHN is a full member of the Protestant Church in Germany (EKD), and is based on the teachings brought forward by Martin Luther during the Reformation.

It is a united church, combining both Calvinist and Lutheran traditions.

The territory of the EKHN includes the territories of the former People's State of Hesse and the Prussian Wiesbaden Region, which now form the southern and western part of the German federal state of Hesse and portions of the German federal state of Rhineland-Palatinate (Rhenish Hesse).

The church ran an Evangelical Academy in Arnoldshain [de], which was moved to Frankfurt in 2013.