[8] All species are microscopic and are typically found on dead plant matter where they consume bacteria, yeasts, and fungal spores.

Since protosteloid amoebae eat bacteria, yeasts, and fungal spores in the laboratory, it is thought that they also do this in nature.

[19] Since protosteloid amoebae are microscopic one must bring their substrates, dead plant matter, into the laboratory to find them.

Dead plant matter is placed on the agar surface in a petri plate and allowed to incubate for several days to a week.

This is done by picking up fruiting bodies or spores with a sterilised needle and moving them onto agar in a fresh petri plate that has been smeared with a bacterium or yeast upon which the protosteloid amoeba species has been known to grow.