Additionally, Nakamurella multipartite, currently part of the order Frankiales, also formed a clade with the Pseudonocardiaceae species in 100% of the bootstrap replications of the phylogenetic trees.

This one-amino-acid insertion in UMP kinase serves to both provide a molecular marker for nearly all of the Pseudonocardiaceae and suggests N. multipartite is closely related to this group.

Homologs of the proteins are generally found in N. multipartite and G. obscurus, providing additional evidence of these two species being closely related to the orders Pseudonocardiales and Corynebacteriales.

[3] Pseudonocardiaceae comprises the following genera:[4] The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN).

[5][a] Actinomycetospora Pseudonocardia Sciscionella Actinoalloteichus Actinopolyspora Saccharopolyspora Kutzneria Allokutzneria Streptoalloteichus Lentzea Goodfellowiella Umezawaea Saccharothrix Actinosynnema Kibdelosporangium Actinophytocola Actinokineospora Thermocrispum Haloechinothrix Amycolatopsis Prauserella Saccharomonospora Mycobacteriales